Mai Anh Do

Cell & Gene Therapy Scientist · CAR-T Manufacturing Specialist

A cGMP cell therapy specialist with six years across the full development-to-manufacturing chain — process development, tech transfer, and cleanroom production of autologous and allogeneic CAR-T therapies, most recently as Senior Associate Scientist at Allogene Therapeutics. Now based in Bavaria, Germany, and available for her next role.

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Professional Experience

A track record of excellence in cell and gene therapy

Career Break · Bavaria, Germany

Parental Leave & Relocation to Bavaria

2024 – Present

Relocated the family from San Francisco to Bavaria; parental leave for childcare.

Completed German language course to B1 level (certificate)
BAMF professional language course (B2) starting September 2026
EU citizen — no visa sponsorship required; available full-time

Allogene Therapeutics · South San Francisco, CA, USA

Senior Associate Scientist, Process Development

2021 – 2024

Developed and optimized manufacturing processes for late-phase allogeneic T-cell therapies, spanning multiple cell-processing platforms and transfection methods
Led drug product formulation and developed analytical methods for potency and phenotype characterization
Ran scale-up, comparability, and stability studies to ensure consistent product quality

Stanford University, Cancer Institute · Palo Alto, CA, USA

Associate Scientist, Process Development

2019 – 2021

Led process development for autologous CAR-T cell therapies and tech transfer into cGMP manufacturing for a first-in-human study
Authored batch records, SOPs, manufacturing instructions, and raw-material specifications; partnered with QA, QC, and Regulatory Affairs on IND submissions
Developed and ran analytical assays (multicolor flow cytometry, functional and phenotypic tests) and trained manufacturing teams

Stanford University, Cancer Institute · Palo Alto, CA, USA

Clinical Process Development and Manufacturing Professional II

2018 – 2019

Delivered end-to-end manufacturing of CAR-T investigational products for Phase I/II studies in cGMP cleanrooms as operator, verifier, and trainer
Optimized cell-processing workflows (CliniMACS, LOVO, Nucleofector, Cellaca) and handled deviations, CAPA, and process changes

Santa Clara University · Santa Clara, CA, USA

Laboratory Manager & Graduate Researcher

2016 – 2018

Ran the bioengineering laboratory: lab policies, inventory, equipment and safety training, and mentoring of students
Researched engineered vesicles for drug delivery (cell culture, PCR, confocal microscopy, flow cytometry) — the basis for a patent and multiple publications

Core Competencies

Specialized expertise across the cell therapy development-to-manufacturing chain

Cell Therapy / ATMP

CAR-T process development; autologous and allogeneic workflows

GMP & Manufacturing

cGMP cleanroom manufacturing, aseptic technique, batch documentation, tech transfer

Quality & Compliance

Deviations, CAPA, change control, SOPs and batch records, IND submissions (FDA)

Analytics

Multicolor flow cytometry (CytoFLEX, BD Fortessa, FACSDiva, FlowJo), potency and phenotype assays

Platforms & Instruments

CliniMACS Prodigy, Xuri, Sefia, Sepax, Erbi, MaxCyte, Nucleofector, LOVO, Cellaca

Process & Data

Scale-up, comparability and stability studies, GraphPad Prism

Education

Academic foundation in bioengineering

M.Sc. Bioengineering & Biomolecular Engineering

Santa Clara University, California, USA

June 2018

B.Sc. Biochemistry

Christopher Newport University, Virginia, USA

May 2016

Languages

Multilingual across four languages

German

B1 (certified); BAMF professional language course (B2) from September 2026

English

Native proficiency (U.S. Bachelor's and Master's degrees)

Czech

Native proficiency (schooling in Czech)

Vietnamese

Heritage language, conversational

Selected Publications

Research contributions to the scientific community — View on Google Scholar

Targeted delivery of lysosomal enzymes to the endocytic compartment in human cells using engineered extracellular vesicles

MA Do, D Levy, A Brown, G Marriott, B Lu — Nature Scientific Reports 9 (1), 1-11 (2019)

Orchestrating extracellular vesicle with dual reporters for imaging and capturing in mammalian cell culture

D Levy, MA Do, J Zhang, A Brown, B Lu — Frontiers in Molecular Biosciences

Decoy exosomes as a novel biologic reagent to antagonize inflammation

N Duong, K Curley, A Brown, A Campanelli, MA Do, D Levy, A Tantry — International Journal of Nanomedicine 14, 3413 (2019)

Sequential deletion of CD63 identifies topologically distinct scaffolds for surface engineering of exosomes in living human cells

N Curley, D Levy, MA Do, A Brown, Z Stickney, G Marriott, B Lu — Nanoscale Journal 12 (22), 12014-12026 (2020)