Mai Anh Do

Cell & Gene Therapy Scientist · CAR-T Manufacturing Specialist

A cGMP cell therapy specialist with six years across the full development-to-manufacturing chain — process development, tech transfer, and cleanroom production of autologous and allogeneic CAR-T therapies, most recently as Senior Associate Scientist at Allogene Therapeutics. Now based in Bavaria, Germany, and available for her next role.

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Professional Experience

A track record of excellence in cell and gene therapy

Career Break · Mainburg, Germany

Parental Leave & Relocation to Bavaria

2024 – Present

Relocated the family from San Francisco to Bavaria; parental leave for childcare.

Completed German language course to B1 level (certificate)
BAMF professional language course (B2) starting September 2026
EU citizen — no visa sponsorship required; available full-time

Allogene Therapeutics · South San Francisco, CA, USA

Senior Associate Scientist, Process Development

2021 – 2024

Developed and optimized manufacturing processes for late-phase allogeneic T-cell therapies, spanning multiple cell-processing platforms and transfection methods
Led drug product formulation and developed analytical methods for potency and phenotype characterization
Ran scale-up, comparability, and stability studies to ensure consistent product quality

Stanford University, Cancer Institute · Palo Alto, CA, USA

Associate Scientist, Process Development

2019 – 2021

Led process development for autologous CAR-T cell therapies and tech transfer into cGMP manufacturing for a first-in-human study
Authored batch records, SOPs, manufacturing instructions, and raw-material specifications; partnered with QA, QC, and Regulatory Affairs on IND submissions
Developed and ran analytical assays (multicolor flow cytometry, functional and phenotypic tests) and trained manufacturing teams

Stanford University, Cancer Institute · Palo Alto, CA, USA

Clinical Process Development and Manufacturing Professional II

2018 – 2019

Delivered end-to-end manufacturing of CAR-T investigational products for Phase I/II studies in cGMP cleanrooms as operator, verifier, and trainer
Optimized cell-processing workflows (CliniMACS, LOVO, Nucleofector, Cellaca) and handled deviations, CAPA, and process changes

Santa Clara University · Santa Clara, CA, USA

Laboratory Manager & Graduate Researcher

2016 – 2018

Ran the bioengineering laboratory: lab policies, inventory, equipment and safety training, and mentoring of students
Researched engineered vesicles for drug delivery (cell culture, PCR, confocal microscopy, flow cytometry) — the basis for a patent and multiple publications

Core Competencies

Specialized expertise across the cell therapy development-to-manufacturing chain

Cell Therapy / ATMP

CAR-T process development; autologous and allogeneic workflows

GMP & Manufacturing

cGMP cleanroom manufacturing, aseptic technique, batch documentation, tech transfer

Quality & Compliance

Deviations, CAPA, change control, SOPs and batch records, IND submissions (FDA)

Analytics

Multicolor flow cytometry (CytoFLEX, BD Fortessa, FACSDiva, FlowJo), potency and phenotype assays

Platforms & Instruments

CliniMACS Prodigy, Xuri, Sefia, Sepax, Erbi, MaxCyte, Nucleofector, LOVO, Cellaca

Process & Data

Scale-up, comparability and stability studies, GraphPad Prism

Education

Academic foundation in bioengineering

M.Sc. Bioengineering & Biomolecular Engineering

Santa Clara University, California, USA

June 2018

B.Sc. Biochemistry

Christopher Newport University, Virginia, USA

May 2016

Languages

Multilingual across four languages

German

B1 (certified); BAMF professional language course (B2) from September 2026

English

Professional working proficiency; degrees and working language since 2016

Czech

Native proficiency (schooling in Czech)

Vietnamese

Heritage language, conversational

Selected Publications

Research contributions to the scientific community — View on Google Scholar

Targeted delivery of lysosomal enzymes to the endocytic compartment in human cells using engineered extracellular vesicles

MA Do, D Levy, A Brown, G Marriott, B Lu — Nature Scientific Reports 9 (1), 1-11 (2019)

Orchestrating extracellular vesicle with dual reporters for imaging and capturing in mammalian cell culture

D Levy, MA Do, J Zhang, A Brown, B Lu — Frontiers in Molecular Biosciences

Decoy exosomes as a novel biologic reagent to antagonize inflammation

N Duong, K Curley, A Brown, A Campanelli, MA Do, D Levy, A Tantry — International Journal of Nanomedicine 14, 3413 (2019)

Sequential deletion of CD63 identifies topologically distinct scaffolds for surface engineering of exosomes in living human cells

N Curley, D Levy, MA Do, A Brown, Z Stickney, G Marriott, B Lu — Nanoscale Journal 12 (22), 12014-12026 (2020)